Synthesis of lectins by micromycetes of the genus Alternaria
- DOI
- 10.5922/vestniknat-2026-2-7
- Pages
- 108-118
Abstract
Using the example of 10 micromycete strains, including museum strains of Alternaria alternata, Alternaria solani and Alternaria infectoria, as well as isolates obtained from natural samples (soil, potato tubers, and wheat seeds), the ability of micromycetes of the genus Alternaria to synthesize endogenous and exogenous lectins was established. Micromycetes of the genus Alternaria are capable of synthesizing both exogenous and endogenous lectins. It was established that the lectin activity of the studied Alternaria micromycete strains is significantly lower than that of micromycetes of the genus Fusarium. Nevertheless, it was possible to increase the lectin activity titer of Alternaria micromycetes by 2—8 times through treatment of the erythrocyte surface with neuraminidase. Among the new isolates of Alternaria micromycetes, the strain Alternaria alternata 4 (grain) synthesizing lectins with the highest activity titer (512 units) was selected. To confirm its belonging to the genus Alternaria, a molecular genetic analysis was performed, which demonstrated that the obtained strain belongs to the phylum Ascomycota, class Ascomycetes, subclass Dothideomycetidae, order Pleosporales, family Pleosporaceae, species Alternaria alternata. Comparison of the activity titers of exogenous and endogenous lectins demonstrated the predominance of intracellular lectin synthesis, making these forms the most promising for further research.