2023 Issue №1

The paper studies the data of a comprehensive environmental survey of land plots in several streets (i. e. Basseynaya, Lieutenant Katina and Sportivnaya) of the city of Kaliningrad for their compliance with the requirements for a specially protected natural area of local importance. It is shown that the territory of the indicated forest park zone meets the main criteria for designating a specially protected natural area of local importance. Given the urban environment, the territory has a high level of biological diversity, including regionally rare species; within the boundaries of the study area there are unique natural complexes and objects. This is a promising territory to carry out scientific research, environmental educational activities, ecological touristic activities, and regulated recreation.

In recent decades, interest in halophyte plants has increased due to their high content of biologically active substances with powerful antioxidant, antimicrobial, anti-inflammatory and antitumor properties and promising for the prevention of various diseases. Several species of halophytes grow on the territory of the Kaliningrad region, among which the rare species Spergularia marina (L.) Griseb. is of particular interest, the biological activity and content of secondary metabolites of which have not been studied sufficiently. The purpose of this study was to obtain callus cultures, to study the content of some groups of phenolic compounds and the antioxidant activity of extracts. The study carried out the selection of growth regulators and concentrations that induce callus formation. 19 nutrient media were selected for the induction of S. marina callus cultures. The conducted phytochemical analysis showed a significant content of phenolic compounds and hydroxycinnamic acids, as well as a high level of antioxidant activity of extracts of callus cultures. Out of 19 callus cultures, cultures obtained on Murashige and Skoog nutrient media containing the following combinations of growth regulators are promising for obtaining target secondary metabolites: 0.25 mg/l TDZ and 1 mg/l 2,4-D; 0.1 mg/l TDZ and 1.5 mg/l 2,4-D; 0.25 mg/l TDZ and 0.25 mg/l IBA; 0.5 mg/l TDZ and 0.25 mg/l IBA; 0.25 mg/l KinN and 0.5 mg/l 2,4-D.