IL-1β and IL-8 influence on cytoprotective mechanisms in liver tissue in morbid obesity patients with comorbidities
- Pages
- 108-120
Abstract
Obesity is a global health problem. Non-alcoholic fatty liver disease (NAFLD) in obese patients is 62—93 %, in type 2 diabetes is prevalent and reaches 55.5 %. The development of chronic subclinical inflammation in obesity contributes to the development of oxidative stress and mitochondrial dysfunction, which underlie the pathogenesis of obesity-associated diseases, i. e., type 2 diabetes and NAFLD.
The study included 130 patients with morbid obesity and comorbid diseases (NAFLD and type 2 diabetes). The gene expression level (NRF2, TFAM, AMPK, HSF1, HSP70) was determined by real-time PCR and the detection of protein products was confirmed by Western blot. The concentration of cytokines (IL-1β, IL-8) was measured in plasma by multiplex analysis in flow fluorometry format. It was demonstrated that an increase in the concentration of pro-inflammatory cytokines IL-1β, IL-8 in blood plasma was associated with the development of NAFLD and type 2 diabetes in patients with morbid obesity. It was found that an increase in the expression of HSF1 in liver tissue was positively associated with the presence of steatosis and negatively associated with the expression of AMPK. It was found that a decrease in the expression of NRF2 and TFAM in the liver may indicate mitochondrial dysfunction of hepatocytes. Thus, the key factor contributing to the development of NAFLD and type 2 diabetes in morbid obesity is the disruption of the function of systems that maintain redox balance by suppressing the production of the factors NRF2, TFAM, AMPK, HSF1, HSP70 in liver tissue.