Сравнительная оценка биологического действия нативных и синтезированных пептидов
- DOI
- 10.5922/gikbfu-2023-3-8
- Страницы / Pages
- 106-117
Аннотация
Биологически активные пептиды рассматриваются как средства профилактики и лечения различных заболеваний. Из-за высокой стоимости и трудоемкости процесса выделения нативных пептидов в составе лекарственных препаратов, биологически активных добавок все чаще используются синтезированные пептиды. Цель исследования — подтверждение сходства и биологической активности синтезированных и нативных пептидов. В качестве объектов исследования использованы синтезированные и нативные пептиды молозива коров с условным названием T1.1 и mpT2. Синтез пептидов проводили твердофазным методом. Пептид T1.1 сходен с пептидом «POSSUM_01-POSSUM-C-EMBRYO-2KB», биологическая активность которого не исследована. Пептид mpT2 имеет сходство с антидиабетическим пептидом «LL-16 Alytes obstetricans». Доказано, что синтезированные пептиды по физико-химическим характеристикам не отличаются от природных. Синтезированный и природные пептиды не токсичны. Доказано противодиабетическое действие природного и синтезированного пептида mpT2 на животных с индуцированным сахарным диабетом 2-го типа и антиоксидантная активность синтезированного и природного пептида Т1.1.
Abstract
Biologically active peptides are considered as preventive and therapeutic agents for various diseases. Due to the high cost and complexity of isolating native peptides for use in pharmaceuticals, synthetically produced peptides are increasingly being used in dietary supplements. The aim of the research is to confirm the similarity and biological activity of synthesized peptides compared to native peptides. Synthesized and native peptides from bovine colostrum with the code names T1.1 and mpT2 were used as the objects of the study. The peptides were synthesized using the solid-phase method. Peptide T1.1 is similar to the peptide «POSSUM_01-POSSUM-C-EMBRYO-2KB», the biological activity of which has not been studied. Peptide mpT2 is similar to the anti-diabetic peptide «LL-16 Alytes obstetricans». It has been proven that the synthesized peptides do not differ from natural ones in terms of physical and chemical characteristics. Both synthesized and natural peptides are non-toxic. The anti-diabetic effect of natural and synthesized peptide mpT2 on animals with induced type 2 diabetes and the antioxidant activity of synthesized and natural peptide T1.1 have been demonstrated.
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